Dresden 2014 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 9: Posters: Biotechnology and bioengineering

BP 9.6: Poster

Montag, 31. März 2014, 17:30–19:30, P3

DNA origami nanopores for controlling DNA translocation — Silvia Hernandez Ainsa, Nicholas Bell, Vivek Thacker, Kerstin Göpfrich, Karolis Misiunas, and •Ulrich F Keyser — Cavendish Laboratory, University of Cambridge, JJ Thomson Ave, CB3 0HE, Cambridge, UK

DNA origami nanopores are emerging as sensors for biological molecules [1]. Here, we combine DNA origami structures with glass nanocapillaries to reversibly form hybrid DNA origami nanopores. Trapping of the DNA origami onto the nanocapillary is proven by imaging fluorescently labeled DNA origami structures and simultaneous ionic current measurements of the trapping events [2]. We show three applications highlighting the versatility of these DNA origami nanopores. First, by tuning the pore size we can control the folding of dsDNA molecules. Second, we show that the specific introduction of binding sites in the DNA origami nanopore allows selective detection of ssDNA as a function of the DNA sequence [2]. Third, we are able to use voltage to change the state of our DNA origami nanopores to lower the frequency of DNA translocation and explain this with a mechanical model [3].

[1] N. A. W. Bell et al. DNA origami nanopores. Nano Letters 12(1):512-517, 2012.

[2] S. Hernandez Ainsa et al.DNA origami nanopores for controlling DNA translocation. ACS nano, 7(7):6024-6030 2013.

[3] S. Hernandez Ainsa, et al. Voltage responsive DNA origami nanopores. submitted. 2013.