Regensburg 2004 – scientific programme
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AKB: Biologische Physik
AKB 50: Poster Session "Biological Physics"
AKB 50.99: Poster
Friday, March 12, 2004, 10:30–13:00, B
DNA translocation through a biological nanopore — •Ulrich F. Keyser1, N. Wennersbusch1, D. J. Bonthuis1, N. H. Dekker1, X. S. Ling2, and Cees Dekker1 — 1Molecular Biophysics, Delft University of Technology, Lorentzweg , 2628 CJ Delft, The Netherlands1 — 2Brown University, Providence, USA
We study the translocation of single-stranded DNA (ss-DNA) molecules through a single biological nanopore, viz., an alpha-Hemolysin pore from Staphylococcus aureus assembled within a lipid membrane. The inner diameter of this nanopore is only 1.5 nm and ss-DNA molecules can be transferred one by one. Applying a bias voltage at high salt concentrations gives rise to an ionic current through the nanopore. If a ss-DNA molecule enters the pore the current is partly blocked and the passage of a molecule is detected. We are investigating different types of ss-DNA with various lengths and sequences to study the influence on the translocation speed and current. The experimental results will be compared to recent theoretical models.