Berlin 2005 – wissenschaftliches Programm
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AKB: Biologische Physik
AKB 100: Poster Session I
AKB 100.22: Poster
Samstag, 5. März 2005, 16:45–18:45, Poster TU D
Intramolecular dynamics of semiflexible macromolecules by Fluorescence Correlation Spectroscopy — •Roland G. Winkler1, Simon Keller2, and Joachim O. Rädler2 — 1IFF, Forschungszentrum Jülich, D-52425 Jülich — 2Ludwig-Maximilians-Universität, Geschwister-Scholl-Platz 1, D-80539 München
A theoretical description of the dynamics of DNA molecules and actin filaments in solution as measured experimentally by fluorescence correlation spectroscopy is provided and compared to recent experimental results. Particular attention is payed to the contribution of the intramolecular dynamics to the fluorescence correlation function. Using a semiflexible chain model, a theoretical expression is presented for the fluorescence correlation spectroscopy correlation function. The dependence of this function on various model parameters like chain length, persistence length, and fluorescence label density is discussed. Our investigations show that the intramolecular dynamics provides a significant contribution or even dominates the correlation function as soon as the longest intramolecular relaxation time significantly exceeds the shortest experimentally accessible time.