Berlin 2005 – wissenschaftliches Programm

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AKB: Biologische Physik

AKB 100: Poster Session I

AKB 100.36: Poster

Samstag, 5. März 2005, 16:45–18:45, Poster TU D

Length and Temperature Dependence of Voltage-Driven Single Stranded DNA translocation — •U. F. Keyser, N. M. Wennersbusch, N. H. Dekker, and C. Dekker — Kavli Institute of Nanoscience, Delft University of Technology, The Netherlands

We study the translocation of single stranded DNA (ssDNA) molecules through alpha-hemolysin, a biological nanopore inserted into an artificial lipid bilayer. The stem of the pore has a diameter of only 1.5 nm which only can be passed by ssDNA. With a voltage we drive ssDNA through the pore which leads to a brief blockade of the ionic current. Our experiments show that the translcoation time increases linearly when adding poly(dA) ssDNA with a length ranging from of 20 to 100 bases. We studied also the influence of temperature on the translocation time between 8 and 20 degrees Celsius and find a non linear decrease with increasing temperature. It is possible to unzip double stranded DNA with the local electrical field in the pore. We present first measurements with several different molecules to investigate this effect.