Berlin 2005 – wissenschaftliches Programm

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AKB: Biologische Physik

AKB 100: Poster Session I

AKB 100.65: Poster

Samstag, 5. März 2005, 16:45–18:45, Poster TU D

Refractive Index Measurements of Single Cells — •Susanne Ebert, Kort Travis, and Jochen Guck — Universität Leipzig, Abteilung Physik weicher Materie, Linnéstr. 5, 04103 Leipzig, Germany

Techniques using light for the manipulation of cells and for the measurement of cell properties, such as the optical stretcher and the optical tweezers, require exact knowledge of the cells’ refractive index. For the most effective use of these single cell techniques, a method to measure the refractive index of single cells is also necessary. Such a method has value in its own right for application as an inherent cell marker. In the present work, two such refractive index measurement methods are demonstrated. The first method involves the imaging of forward scattering patterns of cells in a collimated laser beam, and the second method, the measurement of the velocities of cells accelerated by Gaussian laser beams. From this data, the index of refraction is extracted by comparison with exact T-matrix calculations, and a self-consistent model of the dielectric properties of the cell is developed.