Berlin 2005 – wissenschaftliches Programm

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AKB: Biologische Physik

AKB 100: Poster Session I

AKB 100.67: Poster

Samstag, 5. März 2005, 16:45–18:45, Poster TU D

Cell adhesion studies with quantum dot labeled cells on structured surfaces — •Tim Liedl, Stefan Kudera, Wolfgang J. Parak, and Friedrich C. Simmel — Department Physik, LMU München, Geschwister-Scholl-Platz 1, 80539 München

The adhesion properties of various cell lines are investigated using microcontact printing techniques. Complex patterns composed of hydrophobic and hydrophilic regions are stamped onto a glass substrate to probe cell mobility and the minimal area required for cell adhesion. Several cell lines are investigated in co-culture after labeling the individual cell lines with fluorescent colloidal nanocrystals with different colors. Due to their reduced tendency to photobleach, nanocrystals are particularly suitable as long term markers. Microcontact printing is performed with polydimethyl siloxane (PDMS) stamps with octadecyltrichlorosilane as an ink. Master stamps with feature sizes on the order of 1 µm are defined in the negative photoresist SU-8 using standard optical and electron beam lithography.