Berlin 2005 – wissenschaftliches Programm
Bereiche | Tage | Auswahl | Suche | Downloads | Hilfe
AKB: Biologische Physik
AKB 100: Poster Session I
AKB 100.76: Poster
Samstag, 5. März 2005, 16:45–18:45, Poster TU D
On the growth control of epithelial cell populations in-vitro — •Dirk Drasdo1, Jörg Galle1, and Markus Löffler2 — 1Interdisciplinary Center for Bioinformatics, Kreutzstr. 7a, 04103 Leipzig — 2Institute for Medical Informatics, Statistics and Epidemiology, 04103 Leipzig
We present a 3d individual cell based biophysical model to study the effect of normal and malfunctioning growth regulation and control on the spatial-temporal organization of growing cell populations in vitro, ranging from monolayer growth to growing tumor spheroids. The model includes explicit representations of typical epithelial cell growth regulation and control mechanisms, namely (i) a cell-cell contact mediated form of growth inhibition, (ii) cell-substrate contact dependent cell-cycle arrest, and (iii) cell-substrate contact dependent programmed cell death. The model cells are characterized by experimentally accessible biomechanical and cell-biological parameters. We study by variation of these cell-specific parameters and apply selective knock-outs of growth regulation and control mechanisms to investigate how the different mechanisms collectively act together. We show that our simulation studies cover the growth behaviour of epithelial cell populations ranging from stem cell populations up to tumor cell lines in vitro.