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Berlin 2005 – scientific programme

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DY: Dynamik und Statistische Physik

DY 43: Einstein Symposium Brownian Motion, Diffusion and Beyond (SYBM) – Contributed Talks I

DY 43.10: Talk

Tuesday, March 8, 2005, 16:45–17:00, TU H2032

Fluorescence correlation spectroscopy: Probing Brownian motion of molecules in solution — •Jörg Enderlein1, Thomas Dertinger1, and Ingo Gregor21IBI-1, Forschungszentrum Jülich, D-52425 Jülich — 2IBI-2, Forschungszentrum Jülich, D-52425 Jülich

In the last three decades, advances in optical microscopy and photoelectric detectors had made it possible to routinely monitor the Brownian motion of molecules in and out of femtoliter detection volumes in solution. The resulting spectroscopic technqiue, fluorescence correlation spectroscopy (FCS), has found wide applications in biophysics, chemical analysis, and physical chemistry. The presentation focuses on recent advances of FCS concerning precise measurement of diffusion coefficient with multi-focal excitation and detection, as well as the combination of FCS with fluorescence lifetime spectroscopy for monitoring fast inter- and intramolecular processes such as conformational changes and molecular interactions.

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