Regensburg 2007 – scientific programme
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BP: Fachverband Biologische Physik
BP 26: Poster Session II
BP 26.6: Poster
Thursday, March 29, 2007, 17:00–19:30, Poster B
Fluorescence Lifetime Imaging of NAD(P)H in MIN6-cells - Information about cellular metabolism — •Stefan Denicke1, Raluca Niesner1, Bülent Peker1, Ingo Rustenbeck2, and Karl-Heinz Gericke1 — 1Institut. f. Physikalische und Theoretische Chemie, Hans-Sommer-Straße 10, 38106 Braunschweig, Germany — 2Inst. f. Pharmakologie und Toxikologie, TU Braunschweig, Mendelsohnstraße 1, 38106 Braunschweig, Germany
In recent years Fluorescence Lifetime Imaging (FLIM) has been increasingly applied to biomedical problems since they provide additional information compared to intensity measurements. NADPH and NADH are important indicators for cellular metabolism. Since both are fluorescent molecules, it is possible to use FLIM as a non-invasive, nonlabeling technique. NADPH occurs in a free and a protein-bound state in the cell. Both states display different fluorescence lifetimes. Immortalised pancreatic beta-cells (MIN6-cells) were treated with various glucose concentrations and the ratio of free and metabolised NADPH was determined by analysing the cumulative fluorescence decay via a noniterative biexponential method. The calculation time can be decreased by more than an order of magnitude compared to iterative analyses. All experiments were performed on a two-photon laser scanning microscope with FLIM in the time-domain.