Dresden 2009 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 17: Poster II
BP 17.17: Poster
Mittwoch, 25. März 2009, 17:15–19:45, P3
Theoretical description of endosome dynamics — •Jonathan Edward Dawson1, Lionel Foret1,2, Claudio Collinet3, Yannis Kalaidzidis3, Lutz Brusch4, Perla Zerial4, Andreas Deutsch4, Marino Zerial3, and Frank Jülicher1 — 1Max Planck Institute for the Physics of Complex Systems, Dresden, Germany — 2Ecole Normale Supérieure, Laboratoire de Physique Statistique, Paris, France — 3Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany — 4ZIH-Technical University Dresden,Germany
We present a theoretical study to describe the collective dynamics of a population of endosomes in a cell. Endosomes are vesicular structures that form networks that sort and transport cargo molecules internalized into the cell by endocytosis. Endosomes undergo fusion and fission thereby changing their size and cargo content. We develop a mean field theory that describes the time evolution of the distribution of endosomal markers or cargo in the network. We calculate these distributions using both analytical and numerical methods. Experimentally these distributions can be determined using fluorescence microscopy. The steady state distribution of total fluorescence intensity of the cargo molecules shows charasteristic and robust features. Our theory is able to quantitatively reproduce the shape of steady state distributions and their time dependence. We determine the kinetic parameters of the early endosomal network in HeLa cells and provide an explanation for observed power law distributions.