Dresden 2009 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 17: Poster II
BP 17.19: Poster
Mittwoch, 25. März 2009, 17:15–19:45, P3
Vesicle Transport in Guided Neuronal Axons — •Carina Pelzl1,2, Guido Piontek3, Jürgen Schlegel3, Joachim Rädler1,2, and Doris Heinrich1,2 — 1Department of Biophysics, Ludwig-Maximilians-Universität München — 2Center of NanoScience (CeNS) — 3Institut für Allgemeine Pathologie und Pathologische Anatomie der Technischen Universität München, Klinikum rechts der Isar
Cellular vesicle transport is crucial to many physiological and pathological events. Several neuronal diseases like Amyotrophic Lateral Sclerosis (ALS) and Alzheimer's are caused by disrupted transport along microtubules.
This work focuses on the retrograde transport of vesicles in PC12 cells and primary ALS neurons. These systems are interesting for their geometrical simplicity, since the microtubules in an axon are almost parallel. In order to further reduce the possible parameters, we force the axons in a perfectly 1D geometry by guiding dendrite outgrowth along predefined nanostructures.
To analyze the vesicle motion within an axon, we use a recently developed algorithm [1], based on a time-resolved mean square displacement (MSD) analysis, to distinguish between active and passive phases with a high temporal resolution.
In this way we can compare naturally occuring 1D transport in living cells to theoretical models. Furthermore, we aim at investigating degeneracies in ALS neurons.
[1] Arcizet et al., Temporal Analysis of Active and Passive Transport in Living Cells, Phys.Rev.Let, in press