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Dresden 2009 – scientific programme

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BP: Fachverband Biologische Physik

BP 17: Poster II

BP 17.28: Poster

Wednesday, March 25, 2009, 17:15–19:45, P3

Novel PSs for PDT: time-resolved detection of 1O2-phosphorescence allows to determine the PS’s localisation — •Christoph Feest1, Annegret Preuss1, Beate Röder1, and Ursula Simonis21Institut für Physik, Humboldt-Universität zu Berlin — 2Department of Chemistry and Biochemistry, San Fransisco State University

Novel photosensitisers (PS) for Photodynamic Therapy (PDT) have been designed to specifically localize at mitochondria as they play a key role in programmed cell-death (apoptosis). The PSs are based on a Tetraphenylporphyrin core and have specific substitutions that modulate their physico-chemical properties and allow for specific intracellular localisation. The photophysical parameters of all compounds were determined in solution. Additionally, the PDT relevant singlet oxygen (1O2) generation was investigated in vitro using flash-photolysis and time resolved 1O2 luminescence detection. A new setup was successfully used for evaluation of 1O2 consumption during low-dose irradiation of cells. The intracellular localization was investigated in vitro using CLSM and FLIM technique. In the future, the combination of these optical methods for investigation of light-induced photosensitized processes may enable us to precisely determine the intracellular site of the photodynamic action.

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