Dresden 2009 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 17: Poster II
BP 17.40: Poster
Mittwoch, 25. März 2009, 17:15–19:45, P3
Recording of glycolytic oscillations by electrical measurements at planar yeast cell/electrode-interfaces — •Christian Warnke1, Mathias Müller1, Michael Charpentier1, Hartmut Witte1, Thomas Mair2, Marcus J. B. Hauser2, and Alois Krost1 — 1Otto-von-Guericke-Universität Magdeburg, Inst. Exp. Phys., Abt. Halbleiterepitaxie — 2Otto-von-Guericke-Universität Magdeburg, Inst. Exp. Phys., Abt. Biophysik
One example for temporal macroscopic oscillations is glycolysis in yeast cells. For studying and recording the glycolytic oscillations the measurement of the NADH-fluorescence is used as a standard method. An alternative detection method of glycolytic oscillations of yeast cells and yeast extract is the use of impedance measurements by a planar yeast cell/blank electrode interface [1]. This interface was developed further by the isolation of the utilized Ti-Au-electrodes on glass substrates with Ta2O5 and SiO2 layers. As an other alternative approach we used the source-drain-current of an AlGaN/GaN High Electron Mobility Transistor (HEMT) to detect electrical signals from yeast cells. We found oscillations of the electrical measurement parameters with the same temporal dynamics as the glycolytic ones. In order to identify the underlying processes in yeast cells responsible for the electrical signals, we analyzed theses oscillations at different electrical conductivities of the cell membranes.
[1] Reiher, A. et al.: Electrical stimulation of the energy metabolism in yeast cells using planar Ti-Au-Electrode interface, J. Bioenerg. Biomembr. 38 (2006), 143-148.