Dresden 2009 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 17: Poster II
BP 17.8: Poster
Mittwoch, 25. März 2009, 17:15–19:45, P3
Biomembrane adhesion on micropatterned substrates: A tool for thermal fluctuation analysis — •Cornelia Monzel1, Susanne Fenz1, Sabine Dieluweit1, Kheya Sengupta2, and Rudolf Merkel1 — 1Institute of Bio- and Nanosystems 4: Biomechanics, Research Centre Jülich, Germany — 2CINAM/CNRS-UPR3118, Luminy, Marseille, France
Cell adhesion is a complex process involving a manifold of forces. Much is known about the specific binding between biomolecules which affect cell adhesion. However, contributions due to generic interactions or repulsive thermal fluctuations are as yet barely understood. Therefore, we developed a simplified model system which permits us to do quantitative analysis of membrane fluctuations. Here, cell adhesion was mimicked by a system consisting of giant unilamellar lipid vesicles, with the specific binding being mediated by the biotin-neutravidin complex. Micropatterns of adhesion-competent and repulsive areas were produced on glass surfaces by microcontact printing. This technique provided us with the means to confine the membrane in a controlled manner. The adhered vesicle exhibited areas of fluctuating and fixed membrane corresponding to the underlying pattern. From Dual-Wavelength Reflection Interference Contrast Microscopy (DW-RICM) analysis, we reconstructed the membrane height distribution and quantified the membrane fluctuations with nano-metric accuracy. We calculated the fluctuation spectrum and the effective potential in which the membrane fluctuates.