Dresden 2009 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 26: Photobiophysics
BP 26.4: Vortrag
Freitag, 27. März 2009, 11:45–12:00, ZEU 260
Polarisation-dependent Raman measurements of crystallized photosystem II — •Katharina Brose1, Athina Zouni2, Peter Hildebrandt2, Christian Thomsen1, and Janina Maultzsch1 — 1Institut für Festkoerperphysik, Technische Universität Berlin, Hardenbergstrasse 36, 10623 Berlin — 2Institut für Chemie, Technische Universität Berlin, Strasse des 17. Juni 135, 10623 Berlin
Raman spectroscopy is one of the standard methods to analyse the structural and vibrational properties of molecules and solids. In photosynthesis, the energy of light is converted into a separation of charge in the photosystem II reaction center. Using the newest photosystem II (PSII) dimer crystal structure (3.0 Å resolution), in which 11 β-carotene molecules (Car) and 14 lipids are visible in the PSII per monomer [1]. In the reaction center two Car molecules CarD1 and CarD2 are assigned, which are oriented perpendicular to each other. The function of these two carotene molecules in the photosynthesis process is still under debate. Polarisation-dependent Raman measurements are expected to give deeper insights in the structure-function relationship of these two Car molecules in the reaction centre of PSII. In this talk, we will present polarisation dependent Raman measurements on single PSII crystals of PSII.
[1] Loll, B., Kern, J., Saenger, W. Zouni, A., Biesiadka, J. (2005). Towards complete cofactor arrangement in the 3.0 Å resolution structure of photosystem II. Nature 438, 1040-1044.