Dresden 2009 – scientific programme

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BP: Fachverband Biologische Physik

BP 7: Poster I

BP 7.40: Poster

Monday, March 23, 2009, 17:45–20:00, P3

Comprehensive Acquisition and Analysis Software for Optical Tweezers — •Fabian Czerwinski and Lene B. Oddershede — Niels Bohr Institute, Blegdamsvej 17, Copenhagen

Optical tweezers have become a valuable tool in biophysics e.g. for precise detection and manipulation of individual (biological) molecules. We present a comprehensive Labview toolbox for optical tweezers with a photodiode-based detection system. Various incorporated methods allow for calibrating biological objects and optical handles directly [1,2]. Acquisition parameters can be precisely adjusted, leading to reliable feedback modes [3] and minimized noise [4]. Drift and noise are quantified on-the-fly by improved Allan-variance algorithms [5].

The main program is designed in a modular fashion to offer (optionally) independent as well as interconnected control of diode, stage and microfluidics. Further, it also contains support for data-streaming protocols. In order to assure minimal failure and negligible error rates, we utilize programming options such as multicore processing, cache-speed optimization and pipelined register control. The source code is available upon request and under Creative Common License. Future improvements will include an extended readout of image devices to facilitate parallel single-particle tracking and further possibilities for calibration.

[1] Berg-Sorensen et al., Rev Sci Instr (17)594, 2004. [2] Tolic-Norrelykke et al., Rev Sci Instr (77)103101, 2006. [3] Greenleaf et al., PRL (95)208102, 2005. [4] Gibson et al., Opt Exp (16)5958, 2008. [5] Czerwinski, Matlab Central, 21727, 2008.