Dresden 2009 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 8: Developmental Processes
BP 8.8: Vortrag
Dienstag, 24. März 2009, 12:00–12:15, HÜL 186
Early Keratinocyte Differentiation and Epithelial-Tissue Morphogenesis on Micropillar Interfaces — •Simon Schulz1, Thorsten Steinberg2, Eva Muessig2, Jens Ulmer1, Niels Grabe3, Gerda Komposch2, Pascal Tomakidi2, and Joachim P. Spatz1 — 1Biophysical Chemistry, University of Heidelberg, and Max-Planck-Institute for Metals Research, Stuttgart, Germany. — 2Department of Orthodontics and Dentofacial Orthopedics, Dental School, University of Heidelberg — 3Department of Medical Informatics, University of Heidelberg
Proliferation and differentiation of keratinocytes play a crucial role in tissue epithelial tissue integrity. Furthermore connective-tissue fibroblasts are pivotal for epithelial-tissue morphogenesis. The combination of material technologies mimicking different tissues with life sciences can lead to the elucidation of fundamental requirements needed for the cells to properly exert tissue specific functions. We fabricated fibronectin covered polydimethylsiloxane (PDMS) micropillar arrays which can be varied in pillar stiffness, diameter and distance. They are applied as a biomechanical microenvironment for immortalized human gingival keratinocytes (IHGKs) and gingival connective-tissue fibroblasts (GCTFs). Qualitative and quantitative differences in expression of the early keratinocyte differentiation markers keratin 1 and 10 could be observed by varying the pillar distances. We show that co-cultures of GCTFs and IHGKs could also be established. Epithelial equivalents of the IHGKs were grown on these topologically defined environments.