Dresden 2009 – wissenschaftliches Programm
CPP 12.4: Poster
Dienstag, 24. März 2009, 14:00–16:30, P3
Cross-calibration of MRX and micro-CT for analysis of tissue samples after magnetic drug targeting — •Helene Rahn1, Ingrid Hilger2, Lutz Trahms3, and Stefan Odenbach1 — 1Institute of Fluid Mechanics, TU Dresden — 2University Hospital Jena — 3Physikalisch-Technische Bundesanstalt, Berlin
Magnetic Drug Targeting (MDT) and Magnetic Hyperthermia, using the strong influence of a magnetic field on ferrofluids, are being considered as local cancer treatments with the aim of reducing the side effects occuring during conventional cancer methods. The success of MDT and MHT depends on the correct distribution of the iron oxide nanoparticles within the tumour. This distribution can be analyzed by the non-invasive methods X-Ray microcomputed tomography (micro-CT) and magnetorelaxometry (MRX). To enable semi-quantitative analysis of these samples, a calibration with phantoms consisting of magnetic nanoparticles embedded in agarose-gel has been performed. The iron concentrations have been determined by MRX and vary from 2,88 E-5 mg/ml for a dilution of 1:300000 to 8,51 mg/ml for a dilution of 1:3. The same samples have been analysed by micro-CT and the minimum concentrations of these iron particles that can be detected by both methods have been determined and represented in a calibration curve. Based on this cross-calibration of micro-CT and MRX any biological sample with an iron oxide concentration within the range from 0.864 mg/ml to 8.64 mg/ml can be analysed by both methods. From both techniques, that is, the concentration and the volume distribution of these nanoparticles will be presented.