Dresden 2009 – wissenschaftliches Programm
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O: Fachverband Oberflächenphysik
O 66: Organic, polymeric, biomolecular films – also with absorbates II
O 66.6: Vortrag
Freitag, 27. März 2009, 12:30–12:45, SCH A316
Microscopic and fluorescence spectroscopic studies of organic molecules in contact to surfaces — •Heinrich Südmeyer1, Franz-Josef Schmitt1, Joachim Börner1, Max Schoengen1, Hans-Joachim Cappius2, and Hans Joachim Eichler1 — 1Institut für Optik und Atomare Physik, Technische Universität Berlin, Strasse des 17. Juni 135, 10623 Berlin — 2Laser- und Medizin-Technologie GmbH, Fabeckstrasse 60 - 62, 14195 Berlin
Specific amino acids contained in proteins show autofluorescens if excited in UV. Therefore the use of fluorescence markers is not necessary for high resolved fluorescence microscopy. On the other hand it is often necessary to immobilize proteins. But the photophysical and biochemical properties of organic molecules change due to surface contact. To understand these properties the change of protein conformations due to the surface contact must be analyzed. Combined microscopic and time correlated single photon counting methods (TCSPC) are very promising because the fluorescence depends on the conformational structure of the proteins and allows to analyze different quenching mechanisms. With TCSPC energy transfer processes between neighbouring amino acids, neighbouring molecules and between molecules and the surface can be analyzed. Due to the fluorescence kinetics it is also possible to separate the fluorescence of spectrally similar fluorescence emitters.