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Regensburg 2010 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 32: Posters: Physics of Cells

BP 32.32: Poster

Donnerstag, 25. März 2010, 17:15–20:00, Poster B1

Calcium Signaling upon mechanical stimulus in the Optical Stretcher — •Markus Gyger and Josef Käs — Universität Leipzig, Fakultät für Physik und Geowissenschaften, Institut für Experimentelle Physik I, Linnéstraße 5, 04103 Leipzig, Germany

Under physiological conditions many cells must react to mechanical stimuli. Calcium is one of the most important second messengers and is involved in most of the known mechano-activated cell responses. There are indications that during tumorgenesis the calcium signaling of a cell changes most likely leading to suppression of apoptosis and altered gene expression. The calcium influx can be made visible by appropriate fluorescent dyes, also chelating agents, quenching internal calcium signals as well as external calcium, are available. This provides a broad range of tools for the investigation of effects of calcium on the response of the cell to an external stimulus. The Optical Stretcher is a tool to probe global mechanical behavior of single cells in suspension. Cells are trapped by two anti-parallel laser beams. By increasing the laser power the momentum transferred to the cell surface causes visible deformations. Some cells, especially cancer cells, seem to respond actively to these deformations sometimes even resulting in a contraction of the cell relative to its initial, undeformed state counteracting the applied force. This raises interesting questions regarding the mechanisms by which cells register and respond to the applied forces. The aim of the presented work is to investigate the dependence of calcium influx on the forces applied to the cell surface in order to gain insight into the mechanisms of active responses to stretching.

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