Hannover 2013 – scientific programme
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MO: Fachverband Molekülphysik
MO 14: Poster 1: Cluster, Femtosecond Spectroscopy
MO 14.8: Poster
Tuesday, March 19, 2013, 16:00–18:30, Empore Lichthof
Ultrafast mid-infrared spectroscopy of sensory rhodopsin II from Natronomonas pharaonis — •Christian Kijatkin, Kristin Springfeld, Johann Klare, Heinz-Jürgen Steinhoff, and Mirco Imlau — School of Physics, Osnabrück University, Germany
A multitude of several rhodopsins offer functional properties, which are necessary for the use as pumps, sensors or even molecular phototaxis [1]. Transient mid-infrared vibrational spectroscopy is performed to optain more information of the light-induced trans-cis isomerization process of sensory rhodopsin II from Natronomonas pharaonis (NpSRII). We use femtosecond laser pulses with wavenumbers in the fingerprint region of the chromophore NpSRII between 1180 cm−1 and 1206 cm−1 and of the etyhlenic stretch vibrations between 1530 cm−1 and 1572 cm−1.
The transmission of mid-infrared τ≈150 fs pulses is detected by a multichannel MCT detector as a function of time delay to an intense VIS pump pulse of τ≈100 fs pulse duration. We present our results on the studies of the light-induced changes of such vibrational modes on the 100 fs time scale compared to FTIR difference spectra.
Financial support by the DFG (IM 37/5, INST 190/137-1) is gratefully acknowledged.
[1] J. P. Klare et al., Bioenergetics, 45, 73-122 (2008)