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Dresden 2014 – scientific programme

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BP: Fachverband Biologische Physik

BP 13: Posters: DNA/RNA and related enzymes

BP 13.8: Poster

Tuesday, April 1, 2014, 09:30–12:30, P1

Experiments of DNA-Ligand-Complexes with Optical and Magnetic Tweezers — •Ying Wang1, Susan Haji Samo1, Andy Sischka1, Helene Schellenberg1, Katja Tönsing1, Thomas Jany2, Thorsten Glaser2, and Dario Anselmetti11Experimental Biophysics and Applied Nanoscience, Bielefeld University, 33615 Bielefeld, Germany — 2Lehrstuhl für Anorganische Chemie I, Bielefeld University, 33615 Bielefeld, Germany

We investigated the specific binding of Co-, Cu- and Ni-dinuclear metal complexes (DNMCs) to dsDNA with optical (OT) and magnetic tweezers (MT). DNMCs bind to the DNA backbone, hydrolyse phosphate esters (cutting functionality) and thus prevent the particular DNA segment from being replicated or transcribed. In the OT stretching experiments, we have observed force peaks, suggesting knot or coil formation of DNA entanglements, since the aromatic groups of neighbouring DNMCs can interact with each other via π-stacking. Detecting these complexations have proved the binding of DNMCs to DNA. In twisting experiments with MT, we have evidenced that 200µM Cu-DNMC shows cutting functionality. Because of the created nicks, the DNA molecule can no further be twisted. Combining with molecular recognition techniques DNMCs could be utilized on medical applications as a possible drug dealing with cancers in the future.

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