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BP: Fachverband Biologische Physik

BP 6: Posters: Membranes and vesicles

BP 6.6: Poster

Monday, March 31, 2014, 17:30–19:30, P3

Infrared mapping of membrane proteins with 30 nm lateral resolution — Iban Amenabar¹, •Elmar Hassan Hubrich², Joachim Heberle², and Rainer Hillenbrand¹ — ¹CIC nanoGUNE Consolider, 20018 Donostia-San Sebastián, Spain — ²Experimental Molecular Biophysics, Department of Physics, Freie Universität Berlin, 14195 Berlin, Germany

Infrared spectroscopy is a common technique to study proteins. However, the diffraction limit prevents resolution on the nanometer scale. Using Fourier transform infrared nanospectroscopy (nano-FTIR), we were able to measure membrane proteins with a lateral resolution of 30 nm.

Nano-FTIR combines scattering-type scanning near-field optical microscopy (s-SNOM) and Fourier transform infrared spectroscopy (FTIR). A metalized tip of an atomic force microscope (AFM) is illuminated with a broadband infrared laser and the backscattered light is analyzed by a Fourier transform infrared spectrometer.