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BP: Fachverband Biologische Physik

BP 13: Posters: Imaging and Superresolution Optical Microscopy

BP 13.13: Poster

Monday, March 16, 2015, 17:30–19:30, Poster A

Improvements for Stochastic Optical Fluctuation Imaging (SOFI): Sub-pixel super-resolution images with a conventional wide-field microscope — •Simon Christoph Stein, Anja Huss, and Jörg Enderlein — Drittes Physikalisches Institut, Georg-August Universität Göttingen, Deutschland

The last decade has seen a rapid evolution of a wide array of new super-resolution microscopy techniques which are by now widely available and applied in the life sciences. Among these different techniques, super-resolution optical fluctuation imaging (SOFI) stands out due to its algorithmic and experimental simplicity, requiring only the rapid recording, with a conventional wide-field setup, of the intensity fluctuations from a sample which is labeled with blinking emitters.

The visual fidelity of SOFI, however, is limited by the finite size of the camera's pixel grid. We present a new approach for creating sub-pixel resolution images which is completely artifact-free and straightforward to implement, keeping the simplicity of the original algorithm.

Furthermore we show how an estimate for the PSF of an optical system can be extracted from the raw movie of fluctuating emitters. The knowledge about the PSF is used for post-processing deconvolution, enhancing the quality of SOFI images.