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BP: Fachverband Biologische Physik

BP 13: Posters: Imaging and Superresolution Optical Microscopy

BP 13.15: Poster

Monday, March 16, 2015, 17:30–19:30, Poster A

Image Scanning Microscopy — •Jörg Enderlein — 3. Physikalisches Institut, Georg-August-Universität Göttingen

Recent years have seen an explosion in new advanced and super-resolution methods in fluorescence microscopy, which have culminated in the Nobel Prize in Chemistry for 2014. One of the early methods of these techniques was structured illumination microscopy (SIM), which combines wide-field imaging with a structured illumination for doubling the resolution of a wide-field microscope. An alternative approach is image scanning microscopy (ISM), which combines a conventional confocal laser scanning microscope with a wide-field imaging detector. Although the idea was theoretically proposed by Colin Sheppard already in 1988, its first successful realization was only achieved in 2010. Since then, a whole flood of modifications and implementations of this idea have been published, and by now, even the first commercial implementation of ISM (Airy Scan Microscope by Carl Zeiss Jena) is available. I will present the working principle of ISM and its several implementations and applications.