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Berlin 2015 – scientific programme

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BP: Fachverband Biologische Physik

BP 14: Posters: Neurophysics

BP 14.6: Poster

Monday, March 16, 2015, 17:30–19:30, Poster A

Patterning and Interfacing of Biological Neural Networks — •Norman Shepheard1,2, Stefan Niehörster1, Matthias Schürmann3, Savio Fabretti1, Barbara Kaltschmidt3, Christian Kaltschmidt3, Ulrich Rückert2, Elisabetta Chicca4, and Andy Thomas11Center for Spinelectronic Materials and Devices, Physics Department, Bielefeld University, Germany — 2Cognitronics and Sensor Systems, Bielefeld University, Germany — 3Cell Biology, Bielefeld University, Germany — 4Neuromorphic Behaving Systems, Bielefeld University, Germany

The scale of neural networks reaches from large structures such as the whole human nervous system down to small single neurone networks. To examine the small types of single neurone networks we use a bottom up approach, which consist of two important parts. The first part is the pattern of the cell adhesion layer. The second part is the interface to the network.

To achieve self grown networks we produce an adhesion layer stack made of (3-aminopropyl)triethoxysilane (APTES), glutaraldehyde and poly-lysine. This adhesion layer is patterned via the uv-lithographic "lift-off" technique. The hyppocampal mouse neurones are cultured on top of the adhesion layer. Our results show that the neurones adhere to the poly-lysine and aligning themselves with the pattern. The adhesion pattern is designed to fit to the electrode layout of a multi electrode array, which interfaces the networks.

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