DPG Phi
Verhandlungen
Verhandlungen
DPG

Berlin 2015 – scientific programme

Parts | Days | Selection | Search | Updates | Downloads | Help

BP: Fachverband Biologische Physik

BP 2: Neurophysics I

BP 2.6: Talk

Monday, March 16, 2015, 10:45–11:00, H 1058

Synthetic Neuronal Networks on Glass Using Topological and Chemical Cues — •Andreas Schlegel1, Aune Koitmäe1, Paul Gwozdz1, Jann Harberts1, Christian Heusinger1, Gabriele Loers2, and Robert H. Blick11Center for Hybrid Nanostructures (CHYN) and Institute of Nanostructure and Solid State Physics, University of Hamburg, Germany — 2Center for Molecular Neurobiology Hamburg, University Medical Center Hamburg-Eppendorf, Germany

The understanding of neuronal signal transduction is of interest for research of biological networks. We present a method to achieve directional guidance of neurite outgrowth with the goal of providing synthetic neuronal circuits.

We use glass microstructured with an excimer laser (geometrical confinement). In a second step patterns of Poly-L-Lysine (PLL) are printed onto the glass (chemical guidance). The topological pattern consists of lines with alternating units of containers (diameters ∼ 20 µ m) and channels (width ∼ 4 µm, length 30−200 µm). The distance between the lines varies between 10 and 200 µm. The depth of the structures is 4 µ m. PLL is printed inside the containers to promote cell adhesion.

The neurites prefer to grow within the microstructures over several hundred µ m. Neurons situated inside containers grow neurites along channels and connect to multiple neurons in line over a millimeter range. Crosslinking of neurites between separated lines becomes less common with increasing distance. A transition from partially random behavior to controlled growth is observed.

100% | Mobile Layout | Deutsche Version | Contact/Imprint/Privacy
DPG-Physik > DPG-Verhandlungen > 2015 > Berlin