Berlin 2015 – wissenschaftliches Programm

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CPP: Fachverband Chemische Physik und Polymerphysik

CPP 45: P10: Colloids and Complex Liquids

CPP 45.1: Poster

Mittwoch, 18. März 2015, 10:00–13:00, Poster A

Early stage of liquid-liquid phase separation in protein solutions studied by USAXS — •Fajun Zhang1, Stefano Da Vela1, Michal Braun1, Michael Sztucki2, and Frank Schreiber11Institut für Angewandte Physik, Universität Tübingen, 72076 Tübingen — 2ESRF, Grenoble, France

We present new results on the early stage of liquid-liquid phase separation (LLPS) in protein-salt solutions using the improved USAXS beamline (ID2, ESRF). We use our model system of bovine serum albumin (BSA) with YCl3 [1,2], which shows LLPS and a lower critical solution temperature (LCST) phase behavior and can be rationalized using an ion-activated patchy-colloid model [3]. The USAXS data of sample solutions after a temperature jump exhibit a peak that grows in intensity and shifts to lower q values with time. The characteristic length scale obtained from this scattering peak increases with time as t0.3, which will be further discussed for different temperature jumps as well as sample compositions. The method established in this work can be used to study the arrested spinodal decomposition in protein and colloid systems covering both the correlation length and the local structure. [1] F. Zhang, et al. Phys. Rev. Lett. 101, 148101 (2008) Proteins 78, 3450-3457 (2010). [2] F. Zhang, et al. Soft Matter 8, 1313-1316 (2012) and Faraday Discuss. 159, 313-325 (2012) [3] F. Roosen-Runge, et al. Sci. Rep. 4, 7016 (2014)

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