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Q: Fachverband Quantenoptik und Photonik
Q 39: Nano-Optics II
Q 39.3: Vortrag
Mittwoch, 2. März 2016, 15:00–15:15, f342
Fast and Precise Studying of Dynamical Processes on Live Cell Membranes Using Interferometric Scattering Microscopy (iSCAT) — •Mihail Petev1,2, Richard W. Taylor1, Hawzhin Hozhabrpour1,2, Christian Riess2, and Vahid Sandoghdar1,2 — 1Max Planck Institute for the Science of Light,D-91058 Erlangen, Germany — 2Fredrich-Alexander-Universitat Erlangen-Nurnberg (FAU), D-91058 Erlangen, Germany
By monitoring the diffusion of single transmembrane proteins within the live cell membrane, one gains much important understanding of their subtle and nuanced function and interactions. To monitor all of the rich dynamics of such proteins requires very high spatial and temporal resolution that should be sustained over long duration. These requirements are simply inaccessible by conventional fluorescence microscopes. Using interferometric scattering imaging (iSCAT) and by labeling single transmembrane proteins in the live HeLa cell with a gold nanoparticle, we are able to overcome these limitations.
iSCAT microscopy exploits coherent interference between sample-scattered light and a homodyne reference to measure weakly scattered signals with improved signal-to-noise ratio. The interferometric nature of the imaging is thus sensitive to the fine three-dimensional motion of the gold nano-probe on the cell membrane, which we are able to track with nanometric precision at the fast microsecond time scale. An additional advantage of this approach is that one can also extend it to label-free cell membrane imaging, thus eliminating any marker related effects.