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Hannover 2016 – scientific programme

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SYBO: Symposium Biomedical Optics

SYBO 1: Symposium Biomedical Optics 1

SYBO 1.4: Invited Talk

Thursday, March 3, 2016, 12:30–13:00, e415

Biophotonics - a potential solution to unmet medical needs!? — •Juergen Popp — Leibniz Institute of Photonic Technology, Jena, Germany — Institute of Physical Chemistry & Abbe-Center of Photonics, Friedrich-Schiller University Jena, Germany

Understanding the cause of diseases, early disease recognition, targeted disease treatment, predication of therapeutic response and monitoring treatment success; are the underlying principles of the vision associated with modern biomedicine. In the past years biophotonics has witnessed the development of optical / photonic approaches that are potentially in a position to meet these aforementioned unmet medical needs. In this context spectroscopic approaches like e.g. Raman spectroscopy are especially noteworthy. We will show, that Raman spectroscopy holds great promise as point-of-care approach for a fast identification of pathogens and the determination of their antibiotic resistances, which is crucial for patient*s survival. Furthermore, it will be shown that the combination of Raman approaches with other spectroscopic technologies provides a sensitive and selective diagnostic tool for tissue analysis, i.e. spectral histopathology. We will introduce a combined Raman /FLIM (fluorescence lifetime imaging microscopy) fiber optical probe for in-vivo tissue screening. Furthermore we demonstrate how the combination of CARS (coherent anti-Stokes Raman scattering), SHG (second harmonic generation) and two-photon excited autofluorescence (TPEF) enables the fast and label-free characterization of the morphochemistry of frozen section biopsy specimens and therefore represents high potential for an intraoperative frozen section analysis.

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