Regensburg 2016 – scientific programme

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BP: Fachverband Biologische Physik

BP 48: Posters - Bioimaging and Spectroscopy

BP 48.5: Poster

Wednesday, March 9, 2016, 17:00–19:00, Poster C

Optical setup and algorithms for a fast synchronized dual image acquisition — •Jonas Pfeil¹, Tobias Neckernuss¹, Christoph Koch², and Othmar Marti¹ — ¹Institute of Experimental Physics, Ulm University, Germany — ²Department of Physics, Humboldt University Berlin, Germany

For thickness measurements and 3D reconstruction of microscope images, the most-common approach is using a z-stack of pictures. As this requires a sequential image acquisition of the sample with different focal heights, it is not feasible in time resolved experiments. Therefore we use two synchronized cameras to get a minimal z-stack at well defined time points. To image at two different focal heights, we split the light path with a beam splitter and mount the cameras at different distances from the objective. For adjustment and recording of the images it is necessary to develop a software which is able to capture two cameras at the same time.

The software for the camera control is written in Matlab and C++ for easy maintenance and high flexibility and extensibility. Only documented features of Matlab and only standard C++ methods are used to retain highest possible compatibility across different computation systems and versions. We demonstrate a live asynchronous picture display with two different cameras at the same time for alignment purposes and a blocking synchronous method for a short burst capturing of as many frames as possible.