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BP: Fachverband Biologische Physik

BP 2: Bioimaging and Spectroscopy I

BP 2.2: Vortrag

Montag, 20. März 2017, 10:00–10:15, HÜL 386

(3+1)D SIM + quantitative analysis for ophthalmologic research — •Florian Schock^1,2,3, Gerrit Best^1,3, Nil Celik⁴, Yangyi Wang^2,3, Alena Bakulina⁵, Saadettin Sel⁴, Udo Birk^1,3, Rainer Heintzmann^6,7, Jürgen Hesser⁵, Stefan Dithmar^4,8, and Christoph Cremer^1,2,3 — ¹Institute of Molecular Biology, University of Mainz — ²Institute of Pharmacy and Molecular Biotechnology, University of Heidelberg — ³Kirchhoff Institute for Physics, University of Heidelberg — ⁴Department of Ophthalmology, University-Hospital Heidelberg — ⁵Experimenatal Radiation Oncology, University Medical Center Mannheim, University of Heidelberg — ⁶Institute of Physical Chemistry and Abbe Center of Photonics, University of Jena — ⁷Leibniz Institute of Photonic Technology — ⁸Department of Ophthalmology, HELIOS HSK, Wiesbaden, Germany

While super-resolution-microscopy has become widely available, its application in clinical context is still mostly restricted to cultivated cells. We present the application of (3+1)D(3 excitation/emission spectra) Structured Illumination Microscopy to clinical research on extracted tissues as well as quantitative analysis of over 300 human RPE cells and their granules (intracellular particles) regarding connections to age related macular degeneration. Autofluorescencence behaviour is a characteristic hallmark for several chorioretinal conditions, so SIM offers potential for further clinical imaging at illumination powers that allow application to living patients (ongoing clinical study).