Dresden 2017 – wissenschaftliches Programm
BP 21.12: Poster
Dienstag, 21. März 2017, 14:00–16:00, P1A
Detachment of membrane bound virions by competitive ligand-binding induced receptor depletion — •Nagma Parveen1, Stephan Block2, Vladimir Zhdanov1,4, Gustaf Rydell3, and Fredrik Höök1 — 1Department of Physics, Chalmers University of Technology, Gothenburg, Sweden — 2Department of Chemistry and Biochemistry, Freie Universität Berlin, Berlin, Germany — 3Department of Infectious Diseases, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden — 4Boreskov Institute of Catalysis, Russian Academy of Sciences, Novosibirsk, Russia
Multivalent interactions between virions and receptors in a lipid membrane can be weakened using competitive non-pathogenic ligand binding. In particular, the subsequent binding of such ligands can induce detachment of bound virions, a phenomena of crucial relevance for the development of new antiviral drugs. Focusing on the simian virus 40 (SV40) and recombinant cholera toxin B subunit (rCTB), and using (monosialotetrahexosyl)ganglioside (GM1) as their common receptor in a supported lipid bilayer (SLB), we present the first detailed investigation of this phenomenon by employing the quartz crystal microbalance with dissipation (QCM-D) and 2D single particle tracking (SPT) techniques. Analysis of the QCM-D-tracked release kinetics made it possible to determine the binding strength of a single SV40-GM1 pair. The release dynamics of SV40, monitored by SPT, revealed that a notable fraction of SV40 become mobile just before the release, allowing to estimate the distribution of SV40-bound GM1 receptors just prior to release.