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BP: Fachverband Biologische Physik

BP 21: Posters - Membranes and Vesicles

BP 21.8: Poster

Dienstag, 21. März 2017, 14:00–16:00, P1A

Preparation and characterization of 2D phospholipid and copolymer nanomembranes — •Roland Hillmann¹, Dominic Gilzer², Marlén-Viviane Eickmann¹, Niklas Biere¹, Martina Viefhues¹, Tilman Kottke², and Dario Anselmetti¹ — ¹Experimental Biophysics and Applied Nanoscience, Faculty of Physics, Bielefeld University, Germany — ²Physical and Biophysical Chemistry, Department of Chemistry, Bielefeld University, Germany

We investigated Langmuir-Blodgett (LB) nanomembranes (NM) of UV-polymerized phospholipids and of copolymers as free-standing as well as substrate-supported 2D-systems. As a model for artificial and robust biological membranes, they will serve for various applications, such as for artificial filters or for the functional incorporation of channel proteins. The diacetylene phospholipids (PTPE and DC(8,9)PC) as well as the PB-PEO copolymers were prepared by spreading them at an air-water interface and consecutively transferred to a TEM-grid as well as onto mica and graphite substrates. UV-polymerizing of the phospholipids was performed by a 4W 254nm UV lamp. Successful formation of pore-spanning monolayers up to 2um x 8um was verified by helium-ion-microscopy. Further investigation of the UV-polymerized nanomembranes with attenuated total reflection infrared (ATR-IR) spectroscopy allowed us to monitor the polymerization process.