Berlin 2018 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 5: Systems Biology & Gene Expression and Signalling
BP 5.5: Vortrag
Montag, 12. März 2018, 16:00–16:15, H 1028
Proliferation rate inference with continuous labelling assays — Rode Julian1, Brusch Lutz1, and •Rost Fabian1,2 — 1Technische Universität Dresden, Dresden, Germany — 2Max Planck Institute for the Physics of Complex Systems, Dresden, Germany
Precise estimates of proliferation rates are crucial for quantitative models of the development and maintenance of tissues. Continuous labelling assays are a popular approach to infer proliferation rates in vivo. In these assays, proliferating cells take up a label, e.g. BrdU, when synthesizing DNA for cell division. Intuitively, more cells take up the label per time if they proliferate faster. So far, the experimental and theoretical study of continuous labelling assays focused on the dynamics of the mean labelling-fraction but not on the labelling-fraction distribution dynamics. To study this distribution dynamics, we developed a stochastic model of continuous labelling assays. With the model, we study the effects of cell and sample level noise in the distribution of cell cycle lengths. Using simulated data as ground truth, we show that current inference methods give biased proliferation rate estimates. Therefore, we derive analytical results for the Likelihood for our model that can be used to achieve unbiased estimates of the proliferation rates in vivo.