Berlin 2018 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 9: Postersession I
BP 9.1: Poster
Montag, 12. März 2018, 17:30–19:30, Poster A
Internal Dynamics of Unfolded Apo-myoglobin — •Livia Balacescu1,3, Tobias Erich Schrader1, Andreas Stadler2, and Jörg Fitter2,3 — 1Jülich Centre for Neutron Science, Forschungszentrum Jülich GmbH, Outstation at MLZ, Garching, Germany — 2Jülich Centre for Neutron Science JCNS & Institute for Complex Systems ICS, Forschungszentrum Jülich GmbH, Jülich, Germany — 31.Physikalisches Institut (IA), RWTH Aachen, Aachen, Germany
As a model for the well-characterized protein systems in different folding states we chose apo-myoglobin.[1,2,3] We investigated internal dynamics of its unfolded and folded form on a time-scale up to several hundred nanoseconds and in the nanometer length-scale using neutron spin echo spectroscopy (NSE) [4]. Aggregation state and center of mass diffusion were monitored in parallel with dynamic light scattering. Our first NSE data show a polymer like behavior of the unfolded protein. This indicates that the powerful polymer models may be used for characterization of protein systems.
[1] J. Phys. Chem. B, 2015, 119 (1), 72 [2] J Mol. Biol., 1996, 263(4), 531 [3] J. Am. Chem. Soc., 2014, 136 (19), 6987 [4] Richter D. et al., 2005, Neutron Spin Echo in Polymer Systems. Advances in Polymer Science, vol 174. Springer, Berlin, Heidelberg