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BP: Fachverband Biologische Physik

BP 9: Postersession I

BP 9.32: Poster

Monday, March 12, 2018, 17:30–19:30, Poster A

Nanoscale properties of polymer micro-moulds studied by a combination of AFM and SICM — •Annelie Marx, Regina Lange, Ingo Barke, and Sylvia Speller — University of Rostock, Institute of Physics, 18059 Rostock, Germany

PDMS (polydimethylsiloxane) is a versatile material for creating micro-moulds or stamps. We prepared PDMS moulds as test samples for scanning ion conductance microscopy (SICM) and as prospective substrates for cell adhesion experiments. We focus on the achievable level of detail on the nanoscale by producing moulds from dry eched glass structures exhibiting equidistant grids and pillars of different aspect ratios with vertical side walls. We also report on attempts to produce moulds from metallic nanostructures (Fischer samples) and multichannel plates (MCPs). Both the moulds and the original samples were studied by AFM and SICM. With SICM the topography of a soft sample placed in a (conducting) liquid is measured on the nanoscale, largely avoiding direct forces between the sample and the probe. A decent reproduction quality was obtained on samples with 2 μm deep grooves and unity aspect ratio. We discuss general properties and benefits of PDMS micro-moulds for applications in Biophysics (e.g. with live cells), including the possibility to produce artificial replica by creating a second mould from an initial one.

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