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Berlin 2018 – scientific programme

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CPP: Fachverband Chemische Physik und Polymerphysik

CPP 45: Charged Soft Matter, Polyelectrolytes and Ionic Liquids I

CPP 45.7: Talk

Wednesday, March 14, 2018, 12:45–13:00, C 243

Competing interactions and the formation of dynamic protein clusters — •Anita Girelli1, Michal K. Braun1, Felix Roosen-Runge2, Marco Grimaldo3, Tilo Seydel3, Fajun Zhang1, and Frank Schreiber11Institut für Angewandte Physik, Universität Tübingen, 72076 Tübingen — 2Physical Chemistry, Lund University, Sweden — 3ILL Grenoble, France

Protein clusters are potential precursors for protein crystallization. The question of their formation and transient, dynamic or permanent nature is of high relevance to structural biology and biomedicine. Here we report on protein clusters in bovine serum albumin (BSA) - trivalent salt (YCl3, LaCl3) systems. The protein clusters are most probably held together by ion bridges and precede a condensation regime [1, 2]. The clusters are characterized by dynamic light scattering (DLS). For a molar ratio of salt concentration and protein concentration, cs /cp, equal to 4, we can observe the formation of clusters with a lifetime longer than 1 ms. Based on a comparison with self-diffusion data from neutron back scattering experiments [3], we assume that dynamic clusters form for cs /cp between 2 and 4. Furthermore, SAXS measurements for samples under these conditions demonstrate the competing interactions, which support the formation of dynamic clusters.
Soraruf et al., Soft Matter, 10, 894, 2014
Zhang et al., J. Appl. Cryst., 44, 755, 2011
Grimaldo et al., J. Phys. Chem. Lett., 6, 2577, 2015

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