Regensburg 2019 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 1: Protein structure and dynamics

BP 1.1: Hauptvortrag

Montag, 1. April 2019, 09:30–10:00, H4

Structural dynamics of active membrane transporters as seen by single-molecule techniques — •Thorben Cordes — Physical and Synthetic Biology, LMU Munich, Germany — Molecular Microscopy Research Group, University of Groningen, The Netherlands

Membrane transporters are vital to any living system and are involved in the translocation of a wide variety of different substrates. Despite their importance, all proposed molecular models for transport are based on indirect evidence due to the inability of classical biophysical and biochemical techniques to visualize dynamic structural changes. We recently started to use single-molecule fluorescence microscopy to characterize conformational states and changes in active membrane transporters in vitro to directly observe how different steps in transport are coordinated.[1-4] In my contribution I will first introduce our methodological approach to visualize structural dynamics[5] and accurate distances in biomacromolecules[6] using single-molecule spectroscopy and microscopy. Secondly, I will provide an overview of our mechanistic contributions to the field of primary[1,2,4] and secondary active transporters[3]. These involve various prokaryotic transporters, i.e., ABC importer-related periplasmic binding proteins[1,2], the ABC exporter McjD[4] and the sodium-symporter BetP[3].

[1] Gouridis et al., NSMB 22 (2015) 57-64. [2] van der Velde et al., Nature Communications 7:10144 (2016). [3] Jazi et al., Biochemistry 56 (2017) 2031-2041. [4] Husada et al., EMBO Journal (2018) e100056. [5] Lerner et al., Science 359 (2018) eaan1133. [6] Hellenkamp et al., Nature Methods 15 (2018) 669-676.