Regensburg 2019 – wissenschaftliches Programm
BP 19.1: Hauptvortrag
Mittwoch, 3. April 2019, 15:00–15:30, H11
Self-organized wave-like beating of actin bundles — Marie Pochitaloff1, Mathieu Richard1, Takagi Yasuharu2, Wenxiang Cao3, Enrique De La cruz3, Jim Sellers2, Jean-François Joanny1, Frank Jülicher4, Laurent Blanchoin5, and •Pascal Martin1 — 1Institut Curie, Paris, France — 2NHLBI-NIH, Bethesda, USA — 3Yale University, New Haven, USA — 4MPIPKS, Dresden, Germany — 5CEA, Grenoble
The emergent active behaviors of systems comprising large numbers of molecular motors and cytoskeletal filaments remain poorly understood, even though individual molecules have been extensively characterized. Here, we show in vitro with a minimal acto-myosin system that flagellar-like beating emerges naturally and robustly in polar bundles of filaments. Using surface micro-patterns of a nucleation-promoting factor, we controlled the geometry of actin polymerization to produce thin networks of parallel actin filaments. With either myosin Va or heavy-mero myosin II motors added in bulk, growing actin filaments self-organized into bundles that displayed periodic wave-like beating resembling those observed in eukaryotic cilia and flagella. We studied how varying the motor type or changing the size of the actin bundles influenced the properties of the actin-bending waves. In addition, using myosin-Va-GFP to visualize the motors within the actin bundle, we identified a novel feedback mechanism between motor activity and filament bending. Overall, structural control over the self-assembly process provides key information to clarify the physical principles underlying flagellar-like beating.