Regensburg 2019 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 2: Membranes and vesicles I (joint session BP/CPP)
BP 2.2: Vortrag
Montag, 1. April 2019, 09:45–10:00, H10
Prolonged Phospholipid Bilayer Stability due to Hydration on Porous Silicon: Pore Diameter and Porosity Optimization — Nicolas Moraga1, Marcelo Cisternas1, Diego Diaz1, Rodrigo Catalan1, Maria J. Retamal2, Tomas P. Corrales3, Mark Busch4, Patrick Huber4, Marco Soto-Arriaza2, and •Ulrich G. Volkmann1 — 1Institute of Physics and CIEN-UC, P. Univ. Catolica de Chile, Santiago, Chile — 2Faculty of Chemistry and CIEN-UC, P. Univ. Catolica de Chile, Santiago, Chile — 3Department of Physics, UTFSM, Valparaiso, Chile — 4TUHH, Hamburg, Germany
Study of artificial membranes has become an important way to gain insight into the physical behavior of cell membranes. In this work, porous silicon substrates (pSi) were prepared with different pore diameters and porosities. The substrates were characterized with Field Emission Electron Microscopy. The phospholipid (DPPC) was deposited in high vacuum from the gas phase on the pSi. Film thickness was controlled in-situ using Very High Resolution Ellipsometry (VHRE). Samples were hydrated in air with ultrapure water to assemble the bilayer. Phase transitions were measured with VHRE and Stray Light Intensity during temperature cycles. AFM was used to study morphological changes of bilayers as a function of temperature. Our results show that specific pore diameters and porosities of nanoporous substrates prolong phospholipid bilayer stability due to hydration with water stored in the pores. Acknowledgement: FONDECYT Nos. 3160803 (MJR), 1180939 (UGV), 1171047 (MSA) and 11160664 (TPC), CONICYT Fellowship (MC) and CONICYT-PIA ACT 1409.