Regensburg 2019 – wissenschaftliches Programm
BP 27.6: Vortrag
Donnerstag, 4. April 2019, 16:30–16:45, H10
Efficient hemozoin extraction from Plasmodium falciparum parasites — •Ana Strinic1, Agnes Orban2, Szilvia Mucza2, Petra Molnar3, Beáta Vértessy3, Stephan Krohns1, and Istvan Kezsmarki1,2 — 1Experimental Physics V, University of Augsburg — 2Department of Physics, Budapest University of Technology and Economics — 3Hungarian Academy of Sciences Research Centre for Natural Sciences
Hemozoin crystals are a natural biomarker of malaria infection. A prototypical magneto-optical setup uses magnetic and optical properties of hemozoin for a rapid and cheap, yet sensitive detection of malaria parasites within blood samples. As the parasites mature the volume of the crystallites produced within their food vacuole continuously increases. However, the age distribution of parasites within human blood and cell cultures is not homogenous. Thus, unveiling the relation between their age distribution and the size distribution of the hemozoin crystals may facilitate the monitoring of stage-specific drug actions and target oriented drug testing. Taking advantage of the high sensitivity of the magneto-optical method not only to the crystal concentration but also to their size distribution, we aim to determine the relation of size versus age distribution. This however strongly depends on a successful hemozoin extraction from in vitro cultures. Here, I show, how the crystal extraction process and sample preparation affect the quality of the extracted crystals and the magnitude of the magneto-optical signal. This allows determining the optimal procedure and investigating reactions along the preparation, which reduce the hemozoin concentration.