Regensburg 2019 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 6: Poster I
BP 6.64: Poster
Montag, 1. April 2019, 17:30–19:30, Poster B2
The Influence of Force on the Self-Assembly of Myosin II Minifilaments — •Justin Grewe1,2, Kai Weißenbruch3, Martin Bastmeyer3, and Ulrich S. Schwarz1,2 — 1BioQuant, Heidelberg, Germany — 2Institute for Theoretical Physics, Heidelberg, Germany — 3Karlsruhe Institute of Technology, Germany
Force generation and self-assembly are two central processes in biological systems that usually are considered in separation. However, the signals that activate non-muscle myosin II simultaneously lead to self-assembly into myosin II minifilaments as well as progression of the motor heads through the crossbridge cycle. Here we investigate theoretically the possible effects of coupling these two processes. Our assembly model, which builds upon a consensus architecture of the minifilament, predicts a critical aggregation concentration at which the assembly kinetics slows down dramatically. We validate our model by comparing fluorescence recovery after photobleaching simulated with our model against experimental results. Our model also predicts that increasing actin filament concentration and force both lead to a decrease in the critical aggregation concentration. We suggest that due to these effects, myosin II minifilaments in the cell might be in a supercritical state that can react faster to changing conditions than in solution.