Regensburg 2019 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 7: Bioimaging and biospectroscopy II
BP 7.7: Vortrag
Dienstag, 2. April 2019, 11:30–11:45, H4
Quantitative 3d histology of Alzheimer’s disease by holo-tomography — •Marina Eckermann1, Mareike Töpperwien1, Jasper Frohn1, Thany Bui1, Anna-Lena Robisch1, Franziska Van der Meer2, Christine Stadelmann-Nessler2, and Tim Salditt1 — 1Institute for X-ray Physics, Göttingen University, Göttingen, Germany — 2Institute for Neuropathology, University Medical Center, Göttingen, Germany
Towards quantitative 3d virtual histology of the human brain, we have recently achieved significant progress in reconstructing the neuronal architecture of human cerebella [1], combining propagation-based x-ray phase contrast imaging using laboratory µCT and synchrotron radiation [2]. In this way, we aim at a multi-scale workflow, to automatically identify nanometric pathological alterations from ccm-sized tissue blocks. In [3], nano- and µCT was used to image Aβ plaques in heavy metal stained murine tissue. In the present work, we investigate structural alterations from Alzheimer’s disease (AD) in unstained, paraffin-embedded human hippocampus, hence based on native electron density variations. In control tissue data, we predominantly find intact neurons, characterized by a clear difference in electron density between the cytosol and the nucleus. Furthermore, affected neurons appear overall shrinky, with a perishing nucleus and increased density. Beyond that, we also observe diffuse objects of increased density in specific locations (analysis ongoing work).
[1] Töpperwien et al., PNAS 115, 27 (2018).
[2] Töpperwien et al., Sci. Rep. 7, 42847 (2017).
[3] Massimi et al., NeuroImage 184 (2019).