Regensburg 2019 – wissenschaftliches Programm

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O: Fachverband Oberflächenphysik

O 42: Poster Tuesday: Organic Molecules on Inorganic Surfaces

O 42.12: Poster

Dienstag, 2. April 2019, 18:00–20:00, Poster D

Growth Inhibition of <i>P. Fluorescens<i> Biofilms by Ag/Teflon-Like Thin Films: A Comparative Morphological and Spectroscopic Study — •Maria Chiara Sportelli¹, Ruggiero Quarto¹, Erhan Tütüncü², Antonio Valentini³, Christine Kranz², and Nicola Cioffi¹ — ¹Chemistry Department, University of Bari, V. Orabona, 4-70126 Bari, Italy. — ²Institute of Analytical and Bioanalytical Chemistry, Ulm University, Albert Einstein Allee, 11 * 89081 Ulm, Germany — ³Dipartimento Interateneo di Fisica *M. Merlin*, University of Bari, V. Orabona, 4-70126 Bari, Italy

Ag-Teflon-like (Ag-CFx) composites were obtained by ion beam co-sputtering of polytetrafluoroethylene (PTFE) and Ag targets. AgNPs are already used as antimicrobials in health industry, for food storage, in environmental applications. There is an increasing need of anti-biofouling surfaces and materials, to prevent biofilm formations. Biofilms, i.e. colonies of microorganisms embedded in an extracellular polymeric matrix, are a major cause of healthcare-associated infections, since bacteria are highly resistant to antimicrobials. In this perspective, the inhibition of biofilm growth is a crucial issue in the prevention of bacterial infections. In the present study, Ag-CFx composites were characterized by TEM, AFM, XPS and AAS. They were also deposited onto ZnSe ATR crystals. This approach allowed real-time monitoring of P. fluorescens biofilm growth and inhibition induced by the antimicrobial coating. These findings were corroborated by AFM imaging of bacteria incubated on Ag-CFx films.