Dresden 2020 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 2: Focus: Phase Separation in Biological Systems I (joint session BP/CPP)
BP 2.2: Vortrag
Montag, 16. März 2020, 09:45–10:00, SCH A251
Experimental measurement of the phase diagram of liquid-liquid phase separating proteins and peptides — •Emmanouela Filippidi1,2, Anthony Hyman1, and Frank Jülicher2 — 1Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany — 2Pfotenhauerstrasse 108
Peptides and proteins of a variety of organisms are known to undergo liquid-liquid phase separation to a dense and a dilute phase under certain conditions of temperature, pH, salt and macromolecular concentrations. Our goal is to create and study model peptide systems with sequences inspired by proteins in order to study the effect of amino acid sequence to phase separation. Herein, we will present parallel studies of both a protein, FUS, and simplified peptides of known, repetitive sequences.
As our first step, we will present quantitative measurements of both branches of the binodal curves of the phase diagrams obtained via quantitative phase imaging microscopy. We shall focus on how the multiplicity (multivalency) of pi-cation interactions at constant linear density affects their phase diagrams.