BPCPPDYSOE21 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 11: Poster A: Single Molecule, Multicellular, Bioimaging, Focus Sessions, etc.
BP 11.18: Poster
Montag, 22. März 2021, 16:30–19:00, BPp
Cell Fate Clusters in Inner Cell Mass Organoids Arise from Cell Fate Heredity — •Tim Liebisch1,2, Armin Drusko1,2, Biena Mathew1,3, Ernst Stelzer1,3, Sabine Fischer4, and Franziska Matthäus1,2 — 1GU Frankfurt — 2FIAS — 3BLMS — 4JMU Würzburg
Recently, inner cell mass (ICM) organoids have been published as an in vitro model system towards preimplantational development. ICM organoids mimic the second cell fate decision taking place in in vivo mouse embryos. It was shown that cells of the same fate tend to cluster stronger than expected for the currently hypothesised random cell fate distribution. Three major processes contribute to the cell fate arrangements at the 24 h old and 48 h old ICM organoids or mid and late blastocyst, respectively: chemical signalling; cell sorting process; cell proliferation.
An agent-based model was developed, accounting for cellular interactions, cell growth and division. The model was applied to compare current assumptions of how the ICM neighbourhood is formed. The model supports the hypothesis that initial cell fate acquisition is a stochastically driven process. Subsequently, the observed neighbourhood structures can emerge due to cell fate heredity.
Simulations show that the initial cell differentiation process takes place only during a small time window, during ICM organoid composition. Our results leave little room for cellular signalling believed to be important in cell fate decision. Hence, we are discussing an alternative role of chemical signalling in this process.