BPCPPDYSOE21 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 11: Poster A: Single Molecule, Multicellular, Bioimaging, Focus Sessions, etc.

BP 11.29: Poster

Montag, 22. März 2021, 16:30–19:00, BPp

A general theoretical framework to describe the influence of electric field on Mesechymal stem cell differentiation — •Jonathan Dawson¹, Ursula van Rienen^1,2,4, Poh Soo Lee³, and Revathi Appali^1,4 — ¹Institute of General Electrical Engineering, University of Rostock, Germany — ²Life, Light and Matter, Interdisciplinary Faculty, University of Rostock, Germany — ³Max Bergmann Center for Biomaterials, Institute for Materials Science, Technical University of Dresden, Dresden, Germany — ⁴Ageing of Individuals and Society, Interdisciplinary Faculty, University of Rostock, Germany

Bone regeneration is a highly complex and tightly regulated process which involves concerted and controlled action of human mesenchymal stem cell (hMSC) proliferation and differentiation into osteoblasts. Multiple physiological and environment factors influence the osteogenic differentiation and proliferation of hMSCs. Here we present a quantitative study investigating the influence of external electric field on stem cell dynamics, specifically proliferation and differentiation. In experiments, hMSCs were exposed to a low-frequency electrical field applied via a transformer-like-coupling (TLC). Osteogenic differentiation was quantified by measuring expression levels of cell alkaline phosphate (ALP) activity over time. Our mean-field theory describes the dynamics of a population of ALP stained hMSCs and takes into account cell division, cell differentiation, and intracellular ALP activity. Our results show that the stem cell differentiation rate is electric field dependent, and the proliferation rate is cell-density dependent.