BPCPPDYSOE21 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 11: Poster A: Single Molecule, Multicellular, Bioimaging, Focus Sessions, etc.
BP 11.36: Poster
Montag, 22. März 2021, 16:30–19:00, BPp
Euchromatin reorganisation during transcription resembles active microemulsion — •Rakesh Chatterjee1,2, Hui-Shun Kuan1,2, and Vasily Zaburdaev1,2 — 1Department of Biology, Friedrich-Alexander-Universität Erlangen-Nürnberg, 91058 Erlangen, Germany — 2Max Planck Zentrum für Physik und Medizin, 91058 Erlangen, Germany
During transcription RNA polymerase II (Pol II) attaches and moves along the DNA strand to produce messenger-RNA (mRNA). The selective induction of transcription from DNA into RNA shapes and is being shaped by the chromatin organisation. To investigate this complex interplay, we aim to establish a phenomenological model, which qualitatively mimics the experimental results regarding transcription process in primary cell cultures obtained from zebrafish embryos. Our phenomenological lattice model is based on the framework of microphase separation or microemulsion. DNA, mRNA and Pol II serve as the three basic components similar to the oil-water-surfactant system, which exhibits two and three phase coexistence. Freely diffusing Pol II undergoes chemical transitions reflecting different stages of the transcription process. Similar behaviour can be realised by assuming transient dynamics of the surfactants which switches between active and inactive states. We use lattice model simulations and the correlation function approach to characterises different phases of this three component system. The resulting structures can be understood via the continuum theory that we derive by coarse-graining the lattice model.