BPCPPDYSOE21 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 11: Poster A: Single Molecule, Multicellular, Bioimaging, Focus Sessions, etc.

BP 11.6: Poster

Montag, 22. März 2021, 16:30–19:00, BPp

Optical tweezers and multimodality imaging: a platform for dynamic single-molecule analysis — •Bärbel Lorenz, Ann Mukhortava, and Philipp Rauch — LUMICKS B.V. Amsterdam, Pilotenstraat 51, 1059CH Amsterdam, The Netherlands

The possibility to investigate molecular interactions, structure, and dynamics using single-molecule fluorescence- and force spectroscopybased methods has led to many new insights over the past decades. Here, we present our efforts in establishing the easy and reliable experimental workflow for further enabling discoveries in the field of biology and biophysics using both the combination of optical tweezers with single-molecule fluorescence microscopy (C-Trap). As a proof of concept, we will discuss an overview of the experimental designs and the workflow for combining FRET with an ultra-stable optical trap for studying binding and colocalization dynamics of histones and a helper protein on DNA and observing protein/DNA hairpin folding dynamics. These experiments show that the technological advances in hybrid single-molecule methods can be turned into an easy-to-use and stable instrument that opens up new venues in many research areas.